Pharmacological Evaluation of Reno-protective Effect of Naringin and Hesperidin in Renal I/R Injury in Diabetic Rats

Abstract

Objective:

To evaluate the renoprotective effect of Naringin and Hesperidin in renal ischemia-reperfusion induced renal damage in rat model.

Method:

In the present study, wistar albino rats of either sex weighing 150-250 gm were procured from authorized suppliers. They were divided into two sets, diabetic and non diabetic group. Each set is having seven subgroups, with five animals to each subgroup. They were anesthetized by giving thiopentone sodium (45 mg/kg, i.p,) for surgical procedure to induce ischemia. All the animals in each group underwent 30 min of renal ischemia by performing a unilateral abdominal incision and occluding left renal artery by a knot with a sterile cotton thread at renal pedicel of the left kidney, followed by a 4 h reperfusion by releasing the knot. The drugs Naringin (20, 40 mg/kg) and Hesperidin (20, 40 mg/kg) were administered 10 min before the beginning of reperfusion. Then after 4 h reperfusion, animal was sacrificed and immediately the left kidney was removed, homogenized, centrifuged and supernatant was collected, and same procedure was followed in STZ (45 mg/kg, i.p,) induced diabetic rats. The levels of same biochemical parameters such as catalase (CAT), superoxide dismutase (SOD), malonylaldehyde (MDA) and myeloperoxidase (MPO) were estimated after reperfusion in both the groups.

Results: Naringin and Hesperidin showed significant renoprotective effect by decreasing degree of free radical formation and levels of biochemical stress markers came nearer to normal group compared to I/R control group.

Conclusion: Present study shows that Naringin and Hesperidin shows the renoprotective action through antioxidant mechanism against the damage by oxygen free radicals in reperfusion injury.

Keywords: Ischemia reperfusion injury; free radicals; lipid peroxidation scavenging enzymes; Naringin; Hesperidin.