Cardioprotective effect of Flavonoids, on lipid peroxides and antioxidants in isoproterenol induced myocardial infarcted rats

Abstract

Ischemic heart diseases have been the leading cause of death in both developed and developing countries over the past decades. The aim of the study was to investigate cardioprotective actions of hesperetin and silymarin on lipid peroxidase and antioxidants in normal and isoproterenol induced myocardial infarcted rats. Subcutaneous injection of isoproterenol (150 mg kg-1) to male wistar rats at an interval of 24 h for 2 days showed a significant (p < 0.001) increase in cardiac marker enzymes (ALT, AST), significant decrease in tissue LDH levels, enzymic antioxidants (SOD, GPx, catalase) and nonenzymic antioxidants (GSH, GST). Lipid peroxidative product (MDA) in tissue and serum were significantly (p < 0.001) increased in isoproterenol treated groups. Pretreatment with hesperetin (30, 60, 90 mg kg-1) and silymarin (50,100,150 mg kg-1) to isoproterenol treated rats orally for a period of 37 days daily caused significant effect. Administration of hesperetin and silymarin to normal rats did not have any significant effect on any of the parameters studied. The present findings have demonstrated that the cardioprotective effect of hesperetin and silymarin in isoproterenol induced oxidative damage may be due to an attenuation of oxidative stress, moderate increment in endogenous antioxidant levels and inhibition of lipid peroxidation of membrane, which was further confirmed by histopathological reports.