Identification of Resistance Gene Analogs (RGAs) linked to Powdery Mildew Resistance in Peas.

Abstract

Powdery Mildew caused by Erysiphe pisi, is one of the most common fungal disease in garden peas worldwide. As studied, few pea plants are resistance for powdery mildew and few pea plants are susceptible for powdery mildew disease. All the resistance variety plants contains resistance genes (R-genes), which are the genes in plant genomes that conveys plant disease resistance against pathogens by producing R-proteins. Most of resistance genes encode highly conserved nucleotide binding site and leucine rich repeat structure (NBS-LRR) which helps to isolate specific gene linked to powdery mildew. Degenerative primers based on NBS conserved motif of NBS-LRR resistance protein can be used as molecular RGA markers in PCR amplification for cloning of resistance genes. Later on the screening of genotype with degenerative primers can be done for both resistance genotype and susceptible genotype in order to identifying resistance gene in resistance genotype. Based on resistance gene sequence RGAs were identified, these RGAs would help in identifying marker linked to Powdery mildew resistance and improvise in further Peas Marker Assisted Selection.