Invitro Inhibitory Effect of Polyherbal Formulation on Alpha-Amylase

Abstract

Diabetes Mellitus is a metabolic disorder characterized by high blood sugar level caused due to deficiency of insulin secretion or insulin action. One of the therapeutic approach to treat Type II Diabetes is to lower the postprandial blood glucose level by inhibition of carbohydrate hydrolyzing enzyme such as alpha-amylase. In present investigation polyherbal formulation (PHF) composed of 22 medicinal plants having anti-diabetic property were selected from WHO monographs and evaluated for in vitro alpha-amylase inhibitory activity. Air dried powders of 22 medicinal plants were divided into four categories. One formulation named as(PHF1) was used and prepared by mixing the powders in an optimized ratio of 80:10:5:5.Extracts of PHF1 prepared by soxhlet method using polar and non-polar solvent was subjected to Inhibition assay by using Dinitro salicylic acid (DNS) method and phytochemical constituents in the extract was analyzed qualitatively as well as by GC-MS.The results revealed the presence of glycosides, steroids, terpenoids, saponins, phenols and tannins. In vitro study indicates that PHF1 Hot water extract showed maximum percentage inhibitionof alphaamylase activity. This hot water extract of PHF1 can be effective in lowering postprandial hyperglycemia (PPHG).