Molecular Characterization of Cellulose Degrading Klebsiella variicola RBEB3 (KF036184.1) by 16S rRNA Technique

Abstract

The cellulose degrading bacteria was isolated from soil samples of paper industry waste, cloth industry waste, kitchen waste, garden, earthworm and characterized by morphological and biochemical analysis. The bacteria isolated were grown on Carboxy Methyl Cellulose (CMC) agar media and checked for cellulase activity by adding grams iodine, observing for zone of clearance and identified the organism by gram staining technique. Molecular characterization of cellulose degrading bacteria was determined by the phylogenetic analysis of the 16S rRNA sequence by bioinformatics tools like BLAST. The results showed 99 % resemblance with Klebsiella variicola RBEB3 and the sequence submitted to NCBI with accession number KF036184.1. Cellulase will be used as a possible food supplement or in pharmaceutical industry to degrade cellulose molecule into its monomer called glucose