In Vivo and In Vitro Detection of Anthelmintic Resistance Against Gastrointestinal Nematodes in Sheep

Abstract

The present study was carried out to know the resistance against the drug fenbendazole by in vivo and in vitro method of detection in four sheep farms located in Bangalore and Tumkur districts of Karnataka. Sheep which were not dewormed for the past 2-3 months in a herd and with minimum of 150-200 egg per gram (EPG) count before treatment were selected for the current study. The less value of lower 95% con dence limit indicated the resistance to fenbendazole detected by in vivo test and the ED50 values and LD50 values are more than 0.1 μg/ml and also percentage paralysis at different time intervals indicates the existence of higher number of resistant worms to the drug fenbendazole.