http://13.232.72.61:8080/jspui/handle/123456789/319 Thu, 25 Dec 2025 13:13:22 GMT 2025-12-25T13:13:22Z http://13.232.72.61:8080/jspui/handle/123456789/3342 Title: Modern biotechnology: Origination of paper-based analytical devices Authors: Shilpa, Sivashankar., Salunke, Prasad Abstract: Nowadays most of the biotechnological processes can be performed on benchtop platforms without the need for an expensive, space-consuming equipment. In addition, the sensitivity and specificity of the conventional assays using biotechnological processes can be improved with the recent advancements in materials science and modern technology. One of the materials that aids chromatography, detection of biomarkers with response to a chemical/antibody/protein is “paper”. Hence, analysis of samples i.e., detection, separation, amplification of biomarkers/biomolecules using paper as a substrate can be termed “modern biotechnology”. This is a short review of paper microfluidics has benefitted a wide range of biotechnological/biomedical applications. Sat, 01 Sep 2018 00:00:00 GMT http://13.232.72.61:8080/jspui/handle/123456789/3342 2018-09-01T00:00:00Z http://13.232.72.61:8080/jspui/handle/123456789/3341 Title: Compatibility analysis of 3D printer resin for biological applications Authors: Shilpa, Sivashankar., Alamoudi, Kholod; Agambayev, Sumeyra., Buttner, Ulrich; Khashab, Niveen., Salama, Khaled Nabil Abstract: The salient features of microfluidics such as reduced cost, handling small sample and reagent volumes and less time required to fabricate the devices has inspired the present work. The incompatibility of three-dimensional printer resins in their native form and the method to improve their compatibility to many biological processes via surface modification are reported. The compatibility of the material to build microfluidic devices was evaluated in three different ways: (i) determining if the ultraviolet (UV) cured resin inhibits the polymerase chain reaction (PCR), i.e. testing devices for PCR compatibility; (ii) observing agglutination complex formed on the surface of the UV cured resin when anti-C-reactive protein (CRP) antibodies and CRP proteins were allowed to agglutinate; and (iii) by culturing human embryonic kidney cell line cells and testing for its attachment and viability. It is shown that only a few among four in its native form could be used for fabrication of microchannels and that had the least effect on biological molecules that could be used for PCR and protein interactions and cells, whereas the others were used after treating the surface. Importance in building lab-on-chip/micrototal analysis systems and organ-on-chip devices is found. Mon, 01 Aug 2016 00:00:00 GMT http://13.232.72.61:8080/jspui/handle/123456789/3341 2016-08-01T00:00:00Z http://13.232.72.61:8080/jspui/handle/123456789/3340 Title: Flash μ-Fluidics A Rapid Prototyping Method For Fabricating Microfluidic Devices Authors: Buttner, Ulrich., Shilpa, Sivashankar; Mashraei, Yousof., Agambayev, Sumeyra; Salama, Khaled Nabil Abstract: Microfluidics has advanced in terms of design and structures; however, fabrication methods are time-consuming or expensive relative to facility costs and equipment needed. This work demonstrates a fast and economically viable 2D/3D maskless digital light-projection method based on a stereolithography process. Unlike other fabrication methods, one exposure step is used to form the whole device. Flash microfluidics is achieved by incorporating bonding and channel fabrication of complex structures in just 2.5 s to 4 s and by fabricating channel heights between 25 μm and 150 μm with photopolymer resin. The features of this fabrication technique, such as time and cost saving and easy fabrication, are used to build devices that are mostly needed in microfluidic/lab-on-chip systems. Due to the fast production method and low initial setup costs, the process could be used for point of care applications. Mon, 01 Aug 2016 00:00:00 GMT http://13.232.72.61:8080/jspui/handle/123456789/3340 2016-08-01T00:00:00Z http://13.232.72.61:8080/jspui/handle/123456789/3339 Title: A “Twisted” Microfluidic Mixer with “Turbulent” Fluidic Flow for Wide Range of Flowrate Applications Authors: Shilpa, Sivashankar., Mashraei, Yousof; Agambayev, Sumeyra., Buttner, Ulrich; Salama, Khaled Nabil Abstract: This paper proposes a new “twisted” 3D microfluidic mixer fabricated by a laser writing/microfabrication technique. Effective and efficient mixing using the twisted micromixers can be obtained by combining two general chaotic mixing mechanisms: splitting/recombining and chaotic advection. The lamination of mixer units provides the splitting and recombination mechanism when the quadrant of circles is arranged in a two-layered serial arrangement of mixing units. The overall 3D path of the microchannel introduces the advection. An experimental investigation using chemical solutions revealed that these novel 3D passive microfluidic mixers were stable and could be operated at a wide range of flow rates. This micromixer finds application in the manipulation of tiny volumes of liquids that are crucial in diagnostics. The mixing performance was evaluated by dye visualization, and using a pH test that determined the chemical reaction of the solutions. A comparison of the tornado-mixer with this twisted micromixer was made to evaluate the efficiency of mixing. The efficiency of mixing was calculated within the channel by acquiring intensities using ImageJ software. Results suggested that efficient mixing can be obtained when more than 3 units were consecutively placed. The geometry of the device, which has a length of 30 mm, enables the device to be integrated with micro total analysis systems and other lab-on-chip devices. Wed, 01 Jun 2016 00:00:00 GMT http://13.232.72.61:8080/jspui/handle/123456789/3339 2016-06-01T00:00:00Z